Pet11a ampicillin

lacIq. P T7lac O. BamH I. Nde I. T T7 ampicillin. pBR322 ori. RBS gene 10 leader . pET-11a. 5.7 kb. T7 promoter with lac operator 143 ribosome binding site The ampicillin-resistant NusTag and TrxTag vectors are pET-11a-d. N . plasmids contain the gene for ampicillin resistance (-lactamase) in the same

You have selected for transformants by plating the transformed DNA onto rich media (TCS) containing ampicillin. (The pET11a plasmid contains an ampicillin

Plasmid pET11a-4SS-HEWL from Dr. Harald Schwalbe39s lab contains the insert pBR322origin PstI (754) ORF frame 2 Ampicillin AmpRpromoter AatII (77) The pET 3a, b, c and pET 11a, b, c plasmids have one base pair shift in the BamH I site, . gene for ampicillin resistance and the pBR322 origin of replication This plasmid contains a drug resistant marker for ampicillin resistance (green), the lacI gene (blue), the T7 transcription promoter (red), the lac operator region

pET-11a

16 Sep 2011 Ampicillin. Link to Sequence. Novagen. Features. T7 Promoter Ribosome binding site. Nde I cloning site BamHI cloning site lacI repressor pET11a-link-NGFP is a plasmid vector designed for fusion of a target protein to ampicillin, 35 ugmL kanamycin, 10 uM IPTG and 0.02-0.2 arabinose

bacteria were placed on plates laced with the antibiotic ampicillin (the pET11a plasmid, carries an ampicillin resistance gene) so any bacteria not containing the Plasmid: pET11a EcoRI (5675) AatII (5604) AmpRpromoter PstI (4927) ORF frame 2 Ampicillin pGEX3primer ROP MscI (2760) NruI (2288) EagI (2253) tet